U-Protein Express BV has collaborated with or provided recombinant protein to several scientific organizations resulting in the following publications.

 

2017:
- A human APOC3 missense variant and monoclonal antibody accelerate apoC-III clearance and lower triglyceride-rich lipoprotein levels, S.A. Khetarpal,X. Zeng, J.S Millar, C. Vitali, A.V.H. Somasundara, P. Zanoni, J.A. Landro, N. Barucci, W.J. Zavadoski, Z. Sun, H. de Haard, I.V. Toth, G.M Peloso, P. Natarajan, M. Cuchel, S. Lund-Katz, M.C. Phillips, A.R. Tall, S. Kathiresan, P. DaSilva-Jardine, N.A. Yates and D.J. Rader, Nature Medicine, 2017.


- Recombinant Expression of the Full-length Ectodomain of LDL Receptor-related Protein 1 (LRP1) Unravels pH-dependent Conformational Changes and the Stoichiometry of Binding with Receptor-associated Protein (RAP), C. De Nardis, P. Loumlssl, M. van den Biggelaar, P.K. Madoori, N. Leloup, K. Mertens, A.J. Heck, and P. Gros, Journal of Biological Chemistry, 2017.


- Surrogate Wnt agonists that phenocopy canonical Wnt and β-catenin signalling, C.Y. Janda, L.T. Dang,C. You, J. Chang, W. de Lau, Z.A. Zhong,K.S. Yan, O. Marecic, D. Siepe, X. Li, J.D. Moody, B.O. Williams, H. Clevers, J. Piehler, D. Baker, C.J. Kuo, and K.C. Garcia, Nature, 2017.

 

 

2016:
- Combining somatic mutations present in different in vivo affinity-matured antibodies isolated from immunized Lama glama yields ultra-potent antibody therapeutics, A. Klarenbeek1, C. Blanchetot, G. Schragel, A.S. Sadi, N. Ongenae, W. Hemrika, J. Wijdenes, S. Spinelli, A. Desmyter, C. Cambillau, A. Hultberg, A. Kretz-Rommel, T. Dreier, H.J.W. De Haard, and R.C. Roovers, Protein Engineering, Design and Selection, 2016.

- Structural Mimicry of Receptor Interaction by Antagonistic Interleukin-6 (IL-6) Antibodies, C. Blanchetot, N. De Jonge, A. Desmyter, N. Ongenae, E. Hofman, A. Klarenbeek, A. Sadi, A. Hultberg, A. Kretz-Rommel, S. Spinelli, R. Loris, C. Cambillau, and H. de Haard, JBC, 2016.

- Regulators of complement activity mediate inhibitory mechanisms through a common C3bā€binding mode, F. Forneris, J. Wu, X. Xue, D. Ricklin, Z. Lin, G. Sfyroera, A. Tzekou, E. Volokhina, J.C.M. Granneman, R. Hauhart, P. Bertram, M.K. Liszewski, J.P. Atkinson, J.D. Lambris, P. Gros, The EMBO Journal, 2016.

 

2015:
- Structural basis for inhibition of TLR2 by staphylococcal superantigen-like protein 3 (SSL3), K.J. Koymans, L.J. Feitsma, T.H. Brondijk, P.C. Aerts, E. Lukkien, P. Lössl, K.P. van Kessel, C.J. de Haas1, J.A. van Strijp, and E.G. Huizinga, PNAS, 2015.

 

2014:
- Four individually druggable MET hotspots mediate HGF-driven tumor progression, C. Basilico, A. Hultberg, C. Blanchetot, N. de Jonge, E. Festjens, V. Hanssens, S. Osepa, G. De Boeck, A. Mira, M. Cazzanti, V. Morello, T. Dreier, M. Saunders, H. de Haard and P. Michieli, J Clin Invest, 2014.

- Ensemble refinement shows conformational flexibility in crystal structures of human complement factor D, F. Forneris, B. Tom Burnley, and Piet Gros, Acta Crystallogr. D Biol. Crystallogr., 2014.

- Adenovirus Composition, Proteolysis, and Disassembly Studied by In-depth Qualitative and Quantitative Proteomics, M. Benevento, S. Di Palma, J. Snijder, C.L. Moyer, V.S. Reddy, G.R. Nemerow, and A.J.R. Heck, JBC, 2014.

 

2013:
- Plasma reference value of heart-type fatty acid-binding protein, the earliest available plasma biomarker of acute myocardial infarction, J.F.C Glatz, and R. Mohren, Health, 5 (8), 1206 - 1209.

- Near-Infrared Fluorescence Molecular Imaging of Ductal Carcinoma In Situ with CD44v6-Specific Antibodies in Mice: A Preclinical Study, J.F. Vermeulen, A.S.A. van Brussel, A. Adams, W.P.Th.M. Mali, E. van der Wall, P.J. van Diest, and P.W.B. Derksen, Mol Imaging Biol., 2013, 15(3), 290 - 298.

- Proteome-derived Peptide Libraries to Study the Substrate Specificity Profiles of Carboxypeptidases, S. Tanco, J. Lorenzo, J. Garcia-Pardo, S. Degroeve, L. Martens, F.X. Aviles, K. Gevaert, and P. van Damme, Molecular and Cellular Proteomics, 2013, 12, 2096 - 2110.

- Deep proteome profiling of Trichoplax adhaerens reveals remarkable features at the origin of metazoan multicellularity, J.H. Ringrose, H.W. van den Toorn, M. Eitel, H. Post, P. Neerincx, B. Schierwater, A.F. Altelaar, and A. J. Heck , Nature Communications, 2013, 4, Article 1408.

- Towards Establishing a Rhinoceros-Specific Interferon-Gamma (IFN-γ) Assay for Diagnosis of Tuberculosis, D. Morar, J. Schreuder, M. Mény, P.J.S. van Kooten, E. Tijhaar, A. L. Michel, V.P.M.G. Rutten, Transboundary and Emerging Diseases, (60), Suppl 1: 60 - 60.

- Structures of Wnt-Antagonist ZNRF3 and Its Complex with R-Spondin 1 and Implications for Signaling, W.C. Peng, W. de Lau,P.K. Madoori,F. Forneris,J.C.M. Granneman,H. Clevers, and P. Gros, Plos one, 8(12), e83110.

- The elephant interferon gamma assay: a contribution to diagnosis of tuberculosis in elephants, T. Angkawanish, D. Morar, P. van Kooten, I. Bontekoning, J. Schreuder, M. Maas, W. Wajjwalku, A. Sirimalaisuwan, A. Michel, E. Tijhaar, and V. Rutten, Transboundary and Emerging Diseases, (60), Suppl 1: 53 - 59.

- Structure of Stem Cell Growth Factor R-spondin 1 in Complex with the Ectodomain of Its Receptor LGR5, W.C. Peng, W. de Lau, F. Forneris, J.C.M. Granneman, M. Huch, H. Clevers, and P. Gros, Cell Reports, 3 (6), 1885 - 1892.

 

2012:
- Formation and Structure of a NAIP5-NLRC4 Inflammasome Induced by Direct Interactions with Conserved N- and C-terminal Regions of Flagellin, E.F. Halff, C.A. Diebolder, M. Versteeg, A. Schouten, T.H.C. Brondijk and E.G. Huizinga, JBC, 287, 38460 - 38472.

- Development of a lion-specific interferon-gamma assay, M. Maas, P.J.S. van Kooten, J. Schreuder, D. Morar, E. Tijhaar, A.L. Michel, and V.P.M.G. Rutten, Veterinary Immunology and Immunopathology, 149, (3–4), 292–297.

 

2011:
- Assessment of the Antiviral Properties of Recombinant Porcine SP-D against Various Influenza A Viruses In Vitro, M.L.B. Hillaire, M. van Eijk, S.E. van Trierum, D. van Riel, X. Saelens, R.A. Romijn, W. Hemrika, R.A.M. Fouchier, T. Kuiken, A.D.M.E. Osterhaus, H.P. Haagsman, G.F. Rimmelzwaan, PloS ONE, 6 (9), e25005.

- Introduction of N-linked glycans in the lectin domain of surfactant protein D (SP-D): impact on interactions with influenza A viruses, M. van Eijk, L. Bruinsma, K.L. Hartshorn, M.R. White, M.J. Rynkiewicz , B.A. Seaton, W. Hemrika, R.A. Romijn, B.W. van Balkom, and H.P. Haagsman, JBC, 286, 20137 - 20151.

- Antibody engineering reveals the important role of J segments in the production efficiency of llama single-domain antibodies in Saccharomyces cerevisiae, A. Gorlani, D. Lutje Hulsik, H. Adams, G. Vriend, P. Hermans and T. Verrips, Protein Engineering, Design and Selection, 2012, 25 (1), 39-46.

- Exploring New Proteome Space: Combining Lys-N Proteolytic Digestion and Strong Cation Exchange (SCX) Separation in Peptide-Centric MS-Driven Proteomics, N. Taouatas, S. Mohammed and A.J.R. Heck, Methods in Molecular Biology, 753, 157-167.

 

2010:
 - Insights into complement convertase formation based on the structure of the factor B-cobra venom factor complex, B.J.C. Janssen, L. Gomes, R.I. Koning, D.I. Svergun, A.J. Koster, D.C. Fritzinger, C. Vogel, and P. Gros, EMBO, 28(16), 2469–2478.

- Structures of C3b in Complex with Factors B and D Give Insight into Complement Convertase Formation, F. Forneris, D. Ricklin, J. Wu, A. Tzekou, R.S. Wallace, J.D. Lambris, and P. Gros, Science, 330 (6012), 1816–1820.

- A Novel Disulfide Pattern in Laminin-Type Epidermal Growth Factor-like (LE) Modules of Laminin β1 and γ1 Chains, S. Kalkhof, K. Witte, C.H. Ihling, M.Q. Muller, M.V. Keller, S. Haehn, N. Smyth, M. Paulsson, and A. Sinz, Biochemistry, 49, 8359–8366.

- Molecular Cloning and Functional Characterization of a Zebrafish Nuclear Progesterone Receptor, S.X. Chen, J. Bogerd, Á. García-López, H. de Jonge, P.P. de Waal, W.S. Hong, and R.W. Schulz, Biology of Reproduction, 82 (1), 171-181.

- Clathrin is essential for meiotic spindle function in oocytes, J.J. Holzenspies, B.A.J. Roelen, B. Colenbrander, R.A. Romijn, w. Hemrika, W. Stoorvogel and T. van Haeften, Reproduction, 140, 223-233.

- The influenza A virus hemagglutinin glycosylation state affects receptor-binding specificity, R.P. de Vries, E. de Vries, B.J. Bosch, R.J. de Groot, P.J.M. Rottier, and C.A.M. de Haan, Virology, 403, 17-25.

- Studies in zebrafish reveal unusual cellular expression patterns of gonadotropin receptor messenger ribonucleic acids in the testis and unexpected functional differentiation of the gonadotropins, A. Garcia-Lopez, H. de Jonge, R.H. Nobrega, P.P. de Waal, W. van Dijk, W. Hemrika, G.L. Taranger, J. Bogerd, and R.W. Schulz, Endocrinology, 151 (5), 2349-2360.

- Purification of parvalbumin from Carp: A protocol that avoids heat-treatment, S.J. Koppelman, R.A. Romijn, H.H.J. de Jongh, J.A. Nordlee, S. Piersma, M. Hessing and S.L. Taylor, Journal of food Science, 75 (3), T49-T56.

- Molecular Cloning and Functional Characterization of a Zebrafish Nuclear Progesterone Receptor, S.X. Chen, J. Bogerd, Á. García-López, H. de Jonge, P.P. de Waal, W.S. Hong, and R.W. Schulz, Biology of Reproduction, 82 (1), 171-181.

 

2009:
 - Structural and functional implications of the alternative complement pathway C3 convertase stabilized by a staphylococcal inhibitor, S.H.M. Rooijakkers, J. Wu, M. Ruyken, R. van Domselaar, K.L. Planken, A. Tzekou, D. Ricklin, J.D. Lambris, B.J.C. Janssen, J.A.G. van Strijp and P. Gros,
Nature Immunology, 10 (7), 721-729.

- Oestrogen-induced androgen insufficiency results in a reduction of proliferation and differentiation of spermatogonia in the zebrafish testis, P.P. de Waal, M.C. Leal, Á. García-López, S. Liarte, H. de Jonge, N. Hinfray, F. Brion, R.W. Schulz, and J. Bogerd, Journal of Endocrinology, 202, 287-297.

 - Staphylococcal SSL5 inhibits leukocyte activation by chemokines and anaphylatoxins, J. Bestebroer, K.P.M. van Kessel, H. Azouagh, A.M. Walenkamp, I.G.J. Boer, R.A. Romijn, J.A.G. van Strijp and C.J.C. de Haas, Blood, 113 (2), 328-337.

- Staphylococcal Superantigen-like10 Inhibits CXCL12-Induced Human Tumor Cell Migration, A.M.E. Walenkamp, I.G.J. Boer, J. Bestebroer, D. Rozeveld, H. Timmer-Bosscha, W. Hemrika, J.A.G. van Strijp and C.J.C. de Haas, Neoplasia, 11 (4), 333-344.

- Insights into complement convertase formation based on the structure of the factor B-cobra venom factor complex, B.J.C. Janssen, L. Gomes, R.I. Koning, D.I. Svergun, A.J. Koster, D.C. Fritzinger, C. Vogel and P. Gros, EMBO, 28, 2469-2478.

- Structure and functional relevance of the Slit2 homodimerization domain, E. Seiradake, A.C. von Philipsborn, M. Henry, M. Fritz, H. Lortat-Jacob, M. Jamin, W. Hemrika, M. Bastmeyer, S. Cusack and A.A. McCarthy, EMBO, 10, 736-741.

 

2008:
 - cGMP-binding Prepares PKG for Substrate Binding by Disclosing the C-terminal Domain, V. Alverdi, H. Mazon, C. Versluis, W. Hemrika, G. Esposito, R. van den Heuvel, A. Scholten, A.J.R. Heck,
Journal of Molecular Biology, 104 (38), 14923-14928.

 - Chicken lung lectin is a functional C-type lectin and inhibits haemagglutination by influenza A virus, A. Hogenkamp, N. Isohadouten, S.S.N. Reemers, R.A. Romijn, W. Hemrika, M.R. White, B. Tefsen, L. Vervelde, M. van Eijk, E.J.A. Veldhuizen, and H.P. Haagsman, Veterinary Microbiology, 130, 37-46.

 - Platelet activation by dimeric beta2-glycoprotein I requires signaling via both glycoprotein Ibalpha and apolipoprotein E receptor 2', R.T. Urbanus, M.T. Pennings, R.H. Derksen, and Ph.G. De Groot,  Journal of Thrombosis and Haemostasis, 6 (8), 1405 - 1412.

 - In vitro and in vivo bioactivity of recombinant canine hepatocyte growth factor, B. Arends, B. Spee, G. Hoffmann, G.E.G. Jansen, E. Slump, E. Auriemma, J. IJzer, W. Hemrika, R.A. Romijn, K.H.A. van der Heijden-Liefkens, P.J.A. Sondermeijer, T.S.G.A.M. van den Ingh, L.C. Penning, and J. Rothuizen, The Veternary Journal, 178, 70-77.

 - Crystal structures of TAFI elucidate the inactivation mechanism of activated TAFI; A novel mechanism for enzyme auto-regulation, P.F. Marx, T.H.C. Brondijk, T. Plug, R.A. Romijn, W. Hemrika , J.C.M. Meijers and E.G. Huizinga, Blood, 112 (7), 2803-2809.

 - Cathepsin L functionally cleaves the SARS-CoV class I fusion 1 protein upstream of rather than adjacent to the fusion peptide, B.J. Bosch, W. Bartelink and P. Rottier, Journal of Virology, 82 (17), 8887-8890.

 

2007:
 - Factor B structure provides insights into activation of the central protease of the complement system , F.J. Milder, L. Gomes, A. Schouten, B.J.C. Janssen, E.G. Huizinga, R.A. Romijn, W. Hemrika, A. Roos, M.R. Daha and P. Gros,
Nature Structural & Molecular Biology, 14, 224 - 228.

 - Surface-Plasmon-Resonance-Based Chemical Pteomics: Efficient Specific Extraction and Semiquantitative Identification of Cyclic Nucleotide-Binding Proteins from Cellular Lysates by Using a Combination of Surface Plasmon Resonance, Sequential Elution and Liquid Chromatography-Tandem Mass Spectrometry, N.F.C. Visser, A. Scholten, R.H.H. van den Heuvel, and A.R.J. Heck, ChemBioChem, 8, 298 - 305.

 - The Beta-Defensin Gallinacin-6 Is Expressed in the Chicken Digestive Tract and Has Antimicrobial Activity against Food-Borne Pathogens, A. van Dijk, E.J.A. Veldhuizen, S.I.C. Kalkhove, J.L.M. Tjeerdsma-van Bokhoven, R.A. Romijn and Henk P. Haagsman, Antimicrobal agents and chemotherapy, 51-3, 912-922.

 - Platelets express three different splice variants of ApoER2 that are all involved in signaling, M.T.T. Pennings, R.H.W.M. Derksen, R.T. Urbanus, W.L. Tekelenburg, W. Hemrika, Ph.G. de Groot, Journal of Thrombosis and Haemostasis, 5, 1538 - 1544.

 - Cloning, expression, crystallization and preliminary X-ray analysis of the first two Ig domains from human Roundabout 1 (Robo1), C. Morlot, W. Hemrika, R.A. Romijn, P. Gros, S. Cusack and A.A. McCarthy, Acta Cryst. F63, 689 - 691.

 - Production of Slit2 LRR domains in mammalian cells for structural studies and the structure of human Slit2 domain 3, C. Morlot, W. Hemrika, R.A. Romijn, P. Gros, S. Cusacka, and A.A. McCarthy, Acta Crystallogr D. Biol Crystallography, Sep., 63(Pt 9), 961-968.

 - Structural insights into the Slit-Robo complex, C. Morlot, N.M. Thielens, R.B.G. Ravelli, W. Hemrika, R.A. Romijn, P. Gros, S. Cusack and A.A. McCarthy, PNAS, 104 (38), 14923-14928.


2006:
 - Interaction of ß2-glycoprotein I with members of the low density lipoprotein receptor family, M.T.T. Pennings, M. van Lummel, R.H.W.M. Derksen, R.T. Urbanus, R.A. Romijn, P.J. Lenting and Ph.G. de Groot,
Journal of Thrombosis and Haemostasis, 4, 1680 - 1690.

 - Origin and evolution of the peroxisomal proteome, Toni Gabaldón, Berend Snel, Frank van Zimmeren, Wieger Hemrika, Henk Tabak and Martijn A Huynen, Biology Direct 2006, 1:8 (23 March 2006).

 - Analysis of the cGMP/cAMP Interactome Using a Chemical Proteomics Approach in Mammalian Heart Tissue Validates phingosine Kinase Type 1-interacting Protein as a Genuine and Highly Abundant AKAP, Arjen Scholten, Mee Kian Poh, Toon A. B. van Veen, Bas van Breukelen, Marc A. Vos, and Albert J. R. Heck, Journal of Proteome Research 2006, 5, 1435-1447.

 - Characterization of bitiscetin-2, a second form of bitiscetin from the venom of Bitis arietans: comparison of its binding site with the collagen-binding site on the von Willebrand factor A3-domain, B. Obert, R.A. Romijn , A. Houllier, E.G. Huizinga and J.P. Girma, Journal of Thrombosis and Haemostasis, 4, 1596-1601.

 - VWF A1 domain can adequately substitute for A3 domain in recruitment of flowing platelets to collagen, Arnaud Bonnefoy, Roland A. Romijn, Petra A.H. Vandervoort, Isabel Van Rompaey, Jos Vermylen and Marc F. Hoylaerts, Journal of Thrombosis and Haemostasis, 4, 2151 - 2161.

 - Structure of Complement Component C2a: Implications for Convertase Formation and Substrate Binding, Fin J. Milder, Hans C.A. Raaijmakers, Mitja D.A.A. Vandeputte, Arie Schouten, Eric G. Huizinga, Roland A. Romijn, Wieger Hemrika, Anja Roos, Mohamed R. Daha and Piet Gros, Structure, 14, 1587-1597.

 

2005:
 - The binding site in beta2-glycoprotein I for apoER2' on platelets is located in domain V, M. van Lummel, M.T.T. Pennings, R.H.W.M. Derksen, R.T. Urbanus, B.C.H. Lutters, N. Kaldenhoven, and P.G. de Groot,
JBC, 280(44), 36729-3673.

 

2003:
 - Mapping the collagen-binding site in the von Willebrand Factor A3-domain. R.A. Romijn, Erik Westein, Barend Bouma, Marion E. Schiphorst, Jan J. Sixma, Peter J. Lenting and Eric G. Huizinga,
Journal of Biological Chemistry, 278(17), 15035-15039.


2002:
 - Structures of Glycoprotein Iba and its complex with von Willebrand Factor A1 domain. E.G. Huizinga, S. Tsuji, R.A. Romijn, J.J. Sixma and P. Gros,
Science, 2002, 297, 1176-1179.
 

2001:
 - Identification of the Collagen-binding Site of the von Willebrand Factor A3-domain. R.A. Romijn, B. Bouma, W. Wuyster, P. Gros, J. Kroon, J.J. Sixma and E.G. Huizinga,
Journal of Biological Chemistry, 2001, 276(13), 9985-9991.